- ￥2270
- Sigma-Aldrich
- 进口
- 69892-100G
- 2025年10月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温
- 保质期：
根据瓶身LOT号查询
- 英文名：
MES monohydrate
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
145224-94-8
- 规格：
100G
属性
产品线
BioXtra
质量水平
200
方案
≥99.0% (T)
表单
powder or crystals
有效pH范围
5.5-6.7
pKa (25 °C)
6.1
mp
>300 °C (lit.)
溶解性
H2O: 0.5 M at 20 °C, clear
痕量阴离子
chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg
痕量阳离子
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg
应用
agriculture
diagnostic assay manufacturing
general analytical
microbiology
异质活性
DNase, none detected
NICKase, none detected
RNase, none detected
protease, none detected
SMILES字符串
O.OS(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key
MIIIXQJBDGSIKL-UHFFFAOYSA-N
一般描述
应用
- 调节生长培养基pH 值，有助于进行基因表达分析以理解分子机制
- 细胞成分的固定和成像，以便通过超高分辨率活细胞显微镜研究低渗应力（ hypotonic stress）对细胞结构和功能的生理效应
- 在体内和体外细胞壁应力分析中调节生长培养基的 pH 值至 5.5-7.0
- 作为缓冲剂稳定酶溶液
- 作为PAGE电泳缓冲液的成分
特点和优势
- 适用于生物化学和细胞生物学研究
- 检测过痕量阴离子和阳离子
- 经测试证明重金属污染含量低，确保其适合各种应用
- 在pH 5.5-6.7 (25 °C) 具有缓冲能力，pKa 值6.1 (25 °C)
制备说明
其他说明
灭菌：通过0.2μM过滤器过滤灭菌。不建议对任何磺酸缓冲液进行高温高压灭菌。如果缓冲液必须不含核酸酶，最好先处理水，然后在高温高压灭菌后添加固体缓冲剂。MES溶液高温高压灭菌后，会变成黄色（尽管pH值并未明显变化）。黄色分解产物具体成分未知。
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文献和实验Hypotonic Stress Induces Fast, Reversible Degradation of the Vimentin Cytoskeleton via Intracellular Calcium Release.
The dynamic response of the cell to osmotic changes is critical to its physiology and is widely exploited for cell manipulation. Here, using three-dimensional stochastic optical reconstruction microscopy (3D-STORM), a super-resolution technique, the hypotonic stress-induced ultrastructural changes of the cytoskeleton of a common fibroblast cell type are examined. Unexpectedly, these efforts lead to the discovery of a fast, yet reversible dissolution of the vimentin intermediate filament system that precedes ultrastructural changes of the supposedly more dynamic actin and tubulin cytoskeletal systems as well as changes in cell morphology. In combination with calcium imaging and biochemical analysis, it is shown that the vimentin-specific fast cytoskeletal degradation under hypotonic stress is due to proteolysis by the calcium-dependent protease calpain. The process is found to be activated by the hypotonic stress-induced calcium release from intracellular stores, and is therefore efficiently suppressed by inhibiting any part of the IP3-Ca2+-calpain pathway established in this study. Together, these findings highlight an unexpected, fast degradation mechanism for the vimentin cytoskeleton in response to external stimuli, and point to the significant, yet previously overlooked physiological impacts of hypotonic stress-induced intracellular calcium release on cell ultrastructure and function.
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