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- Sigma-Aldrich
- 进口
- F0291-25UG
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
−20°C
- 保质期：
根据瓶身LOT号查询
- 英文名：
Fibroblast Growth Factor-Basic, human
- 库存：
有现货
- 供应商：
浙江羽翔生物科技有限公司
- CAS号：
106096-93-9
- 规格：
25UG
属性
产品名称
hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
生物来源
human
质量水平
200
重组
expressed in E. coli
方案
≥97% (SDS-PAGE)
表单
lyophilized powder
效能
≤-0.6 ng/mL
分子量
16.0 kDa
包装
pkg of 4X25 μg
pkg of 25 μg
储存条件
avoid repeated freeze/thaw cycles
技术
cell culture | mammalian: suitable
杂质
≤1.00 EU/μg
颜色
white to faint yellow cast
溶解性
water: soluble 0.025 mg, clear, colorless to faintly yellow
UniProt登记号
P09038
储存温度
−20°C
基因信息
human ... FGF2 (2247)
一般描述
应用
外形
分析说明
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文献和实验Development and characterization of Gal KO porcine bone marrow-derived mesenchymal stem cells.
We demonstrated that neonatal porcine bone marrow-derived mesenchymal stem cell (npBM-MSCs) could improve a critical ischemic limb disease in rat model more efficiently compared with human MSCs. However, since porcine MSC presents galactosyl-alpha 1,3-galactose antigen (Gal antigen), MSC could be eliminated by the xenogeneic rejection. Recently, we established Gal knockout (KO) pigs by a technique of the electroporation of the CRISPR/Cas9 system into vitro-fertilized zygotes. In this study, we hypothesized that MSC from the established Gal KO pigs could further improve the efficacy. Before examining the hypothesis, in this study, we have established and characterized bone marrow-derived MSC from the Gal KO adult pigs (apBM-MSCs). Mononuclear cells (MNCs) were isolated from bone marrow cells of both Gal KO adult pigs and wild-type (WT) adult pigs. MNCs were further manipulated to create Gal KO apBM-MSCs and WT apBM-MSCs. Both MSCs were assessed by their surface markers, the capability of differentiation into adipocytes, osteocytes and chondrocytes, grow speed and colony-forming assay. To assess the efficacy of Gal KO apBM-MSCs, angiogenesis-related genes and immunosuppression-related genes were assessed by cytokine stimulation. Gal KO apBM-MSC showed no Gal antigen on their cell surfaces. Both Gal KO apBM-MSCs and WT apBM-MSCs, presented little or no negative surface markers of MSCs, while they presented positive surface markers of MSCs. Furthermore, Gal KO apBM-MSCs were able to differentiate into adipocytes, osteocytes, and chondrocytes as well as WT apBM-MSCs. There was no difference in doubling time between Gal KO apBM-MSCs and WT apBM-MSCs. Interestingly, the colony-forming efficiency of Gal KO apBM-MSCs was about half that of WT apBM-MSC. However, angiogenesis and immunosuppression-related genes were equally upregulated in both Gal KO apBM-MSCs and WT apBM-MSCs by cytokine stimulation. We created and characterized Gal KO apBM-MSCs which showed similar characteristics and cytokine-induced gene upregulation to the WT apBM-MSCs.
) 。 ( 2 ) 提取介质：被收集的植物材料置于提取介质中。提取介质是为稳定亚细胞馏分的混合试剂。依照 Greimer 和 Deltoid [13」所述并稍作改动：2% 阿拉伯树胶（Sigma) 、1.25% Ficoll (Sigma) 、2.5% 硫酸葡萄聚糖（Fluka) 、25 mmol/L Tris-HCl ( pH 7.4)、0.5 mmol/L EDTA、2.5 mmol/L MgCl2、4 mmol/L 正辛醇、8 mmol/L β- 疏基乙醇 ( Sigma) 、6.8 mmol/L 二乙基焦
法是十分常用的蛋白质比色定量方法，很多试剂供应商都提供基于比色皿和微孔板的BCA蛋白定量试剂盒。BCA蛋白定量法相对于其它比色定量法具有操作简单、稳定、灵敏度高等优点。相对于蛋白质的固有的280nm吸收峰检测，BCA检测法提高了蛋白检测灵敏度和特异性，消除了核酸的干扰，核酸干扰在对细胞裂解物或其他生物样品进行蛋白定量时总是一个难以避免的干扰。在碱性条件下，蛋白将Cu2+ 还原为Cu+ ，Cu+ 与BCA 试剂形成紫色络合物，如图1，其吸光值与蛋白浓度成正比。测定在562nm 处的吸收值，并与标准
used: Tetracycline HCl, Tet (Sigma T3383) 12 mg/ ml in 50% ethanol. Use at 3 ug/ml (Tet3) Kanamycin, Kan (Sigma K800) 10 mg/ ml in water. Use at 40 ug/ml (Kan40)
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