- ￥2570
- Signalway Antibody
- AS0073
- 2025年07月14日
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- 保存条件：
Shipped and store at 4°C for 6 months.
- 库存：
100 Assays
Applications：Functional Assay
Description：Detection and Quantification of Hexokinase Activity.
Alternative Names：EC 2.7.1.1; Hexokinase; Hexokinase type I; Hexokinase type II; Hexokinase type III; Hexokinase type IV (glucokinase)
C=AS Number：9001-51-8
Detection Method：Colorimetric
Sample Types：Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids
产品详细信息请点击：Hexokinase Microplate Assay Kit
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文献和实验为催化己糖 6位上由 ATP使之产生磷酸化反应的酶。 EC 2． 7． 1． 1。其反应过程是： 己糖 ATP→己糖 -6-磷酸 ADP 对α－ D葡萄糖的亲和性很高（ Km=1× 10－ 5 M），对其他的己糖也可进行磷酸化。此反应的平衡向右偏移。△ G°’ =4.0千卡。该酶广泛存在于以糖为能源的细胞，但在酵母、肝脏、肌肉、脑等最多。该酶可被结晶化。分子量约为 9万 6千（酵母己糖激酶）。可被 Mg2 、 K 所活化，但可被 HS试剂阻抑
An Enzymatic Microplate Assay for Testing P-Glycoprotein Substrates and Inhibitors
it possible to detect specific interactions between drugs and P-gp. They took into account the existence of multiple binding sites on P-gp to finalize an optimized strategy that involves the assay of the P-gp ATPase stimulated by three reference substrates
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
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