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- 9011S
- 2025年12月24日
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文献和实验Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection
to the sequences of human and rat acetylcholine receptor subunits was immobilized on glass and silicon slides coated by a copolymer of N ,N -dimethylacrylamide, N -acryloyloxysuccinimide, and 3-(trimethoxysilyl) propyl methacrylate, copoly(DMA–NAS–MAPS
A Proteomics Approach to Identify Redox-Sensitive Proteins
-Rad). 10. CB-X Protein Assay kit (G-Biosciences). 11. Reduction buffer: 50 mM Tris–HCl, pH 8.0, 7 M urea, 2 M thiourea, 1% CHAPS, and 1 mM DTT. 12. 11 cm ReadyStrip IPG strips (pH 4–7) (Bio-Rad). 13. SDS-PAGE running buffer (10×): 250
A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
MATERIALS : 1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% newborn calf serum and 0.1% sodium azide) 2. 7-Amino-actinomycin D (7-AAD, e.g., Calbiochem, CA) 3. Human AB serum, heat-inactivated
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