384-well PCR Plate, FS, 50 Pla

Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates

1.Take the 384 well viper plate from the thermocycler. Remove the Silicone Sealing Mat (Axygen Scientific: AM-384-PCR-RD) and place face down on a paper towel and pat to absorb any liquid that may be on it. Add 30 μl 95% EtOH/0.12 M NaOAc

384-well PCR

plates #TF-0384) or 16.3ml per plate, if PCR volume is 40µl/well (maximum for new ABT-384 plates #AB-0937). Add the enzyme last. We were told that Pfu polymerase can destroy primers if PCR mix stored for too long at room temperature

384-well PCR实验方法

plates #TF-0384) or 16.3ml per plate, if PCR volume is 40µl/well (maximum for new ABT-384 plates #AB-0937). Add the enzyme last. We were told that Pfu polymerase can destroy primers if PCR mix stored for too long at room temperature