Falcon®150mmx15mm Not TC-Treat

Chick Chorioallantoic Membrane (CAM) Assay

, under aseptic conditions, onto a nonbinding surface (i.e., bacteriological Petri dish). The polymerization conditions for each substrate were identical; after mixing the growth factors and/or compounds, 40 μl were pipetted onto each mesh in a

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

tip) alternatively:       Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade);       9'' Pasteur pipettes;       70% Ethanol;       Alcohol burner or Bunsen burner;       Sterile plastic Petri dishes (100 x 15 mm);       Sterile

Establishment of a Primary Culture

and remove the chorioallantoic membrane, exposing the embryo.   Identify and carefully remove the embryo by the neck, using a sterile metal hook or a bent glass rod, and place the embryo in a 100mm petri dish containing phosphate buffered