Falcon® 100mmx15mm Not TC-Trea

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

tip) alternatively:       Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade);       9'' Pasteur pipettes;       70% Ethanol;       Alcohol burner or Bunsen burner;       Sterile plastic Petri dishes (100 x 15 mm);       Sterile

ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES

medium, then plate all onto a 10cm dia petri dish containing NeoPMEFs and 10ml medium. Label plate 2. 5. Set up following control plates. a) Take 0.167 ml (2 x 106 cells) original cell suspension _ 10cm dia petri dish containing NeoPMEF's and 10

ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES

. 500 uF 230V then 500 uF 240V 4. ASAP using a pasteur pipette transfer cells from curvette A to a 10 ml tube containing 4 mls medium, then plate 5 x 1 ml cells onto five 10cm dia petri dishes containing NeoPMEF's and 10 ml medium. Label plates