FSRed Nucleic Acid Gel Stain

Silver Stain Protocol

from protein to protein. Caution: nucleic acids are stain. Fresh Solutions 0.02%  Sodium Thiosulfate (Na2S2O3) 0.1% AgNO3 for minigel and 0.15% for large gel. Stored in 4ºC before use. 2% Na2CO3 + 0.04% formaldehide. Prepare fresh. Note: Wear

Denaturing Agarose Gel Electrophoresis of RNA

, such as those from needle biopsies or from laser capture microdissected samples, result in very low yields. In these cases, it may be impossible to spare 200 ng of RNA to assess integrity. Alternative nucleic acid stains, such as SYBR® Gold and SYBR® Green II RNA gel stain

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

gradient sedimentation, and R s was 10.3 nm, from gel filtration. Putting these values in Eq. 7.1b , we find that the molecular weight of SMC protein from B. subtilis is 273,000 Da. From the amino acid sequence, we know that the molecular weight