手机验证
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SK-N-BE(2)C; SK-N-BE-2c; SK-N-BE(2C); SK-N-BE(2c); SK-N-BE2C; SK_N_BE2C; SKNBE2C; SKNBE(2c); BE(2)-C; BE(2)C; Be(2)C; BE2-C; BE2_C; Be2-C; BE2C; Be2C
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充足
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brain; derived from metastatic site: bone marrow
人神经母细胞瘤
人
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神经母细胞样
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brain; derived from metastatic site: bone marrow
复苏发货或干冰冷冻发货
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贴壁
1 x 10^6 cells/vial/T25
细胞名称:BE(2)-C人神经母细胞瘤(STR鉴定正确)
产品货号:C1296
培养条件:MEM+F12+10%FBS+1%PS (配套完全培养基:M0431)
生长特性:贴壁
细胞形态:神经母细胞样
产品规格:1 x 10^6 cells/vial/T25
产品货号 | 细胞名称 | 中文名称 | 生长特性 | 细胞形态 | 产品规格 |
C1001 | SH-SY5Y | 人神经母细胞瘤 细胞 | 半贴半悬 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1077 | Hs683 | 人脑视神经胶 质瘤细胞 | 贴壁 | 成纤维细胞样 | 1 x 10^6 cells/vial/T25 |
C1080 | IMR-32 | 人神经母细胞 瘤细胞 | 贴壁 | 成纤维细胞样 | 1 x 10^6 cells/vial/T25 |
C1150 | SK-N-SH | 人神经母细胞 瘤细胞 | 半贴半悬 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1194 | SK-N-AS | 人神经母细胞 瘤细胞 | 贴壁 | 上皮样 | 1 x 10^6 cells/vial/T25 |
C1212 | SK-N-BE(2) | 人神经母细胞 瘤细胞 | 贴壁 | 神经母细胞样 | 1 x 10^6 cells/vial/T25 |
C1295 | BE(2)-M17 | 人神经母细胞 瘤细胞 | 贴壁 | 神经母细胞样 | 1 x 10^6 cells/vial/T25 |
用户须知:
1、关于货期:除复苏细胞外,承诺货期1周内到(节假日顺延),大多数产品1-3天内发货。细胞产品因需要复苏后进行1-2代传代调整后等待细胞长满,期间进行微生物检测,货期1、2周左右。
2、关于订购:为了保证您的权益,下单前务必联系我司客户服务热线(021-55150076)。
细胞传代:
1.弃去培养液,用PBS洗涤1-2次;
2.加入1.0ml胰酶消化液,37℃消化,显微镜下观察细胞消化情况,若细胞回缩变圆、透亮、轻拍甁壁呈流沙样脱落,则迅速拿回操作台,加入完全培养液,终止消化并轻轻吹打细胞,使其变成单细胞悬液;
3.将细胞收集于离心管中离心1000rmp/5min,弃上清,轻弹管底,将细胞弹散;
4.加入新鲜培养液重悬细胞,进行传代、冻存;
5.如果没有特别说明,建议收到细胞后的第一次传代比例为1:2。
6.注意培养基PH值变化,定期换液(每周2-3次)。
安全性:
所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护。
联系人:胡经理
电话:15921028707(微信同号)
:3407506161
:021-55150076
官网:https://whelab.com
公司邮箱:info@whelab.com
技术邮箱:tech@whelab.com
地址:上海宝山区呼兰西路129号8号楼4层
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