描述
Dynabeads® M-270 Streptavidin the gold standard for isolation and handling of biotinylated nucleic acids, antibodies, or other biotinylated ligands and targets. The very high binding affinity of the streptavidin-biotin interaction (Kd=10-15) is used in a vast number of applications. Benefits and features:
Dynabeads®M-270链霉亲和素——分离和处理生物素化核酸、抗体或其他生物素化配体和靶点的金标准。链霉亲和素-生物素相互作用（Kd=10-15）具有很高的结合亲和力，被广泛应用于许多领域。
优点和特点：
• Direct and fast isolation of any biotinylated molecule
•直接快速分离任何生物素化分子
• 子
• Flexible protocols with gentle and efficient liquid-phase reaction kinetics
•具有温和高效液相反应动力学的灵活协议
• j议
• Very low nonspecific binding of nucleotides and nucleic acids
•核苷酸和核酸的非特异性结合非常低
• Very low aggregation in high-salt hybridization buffers
•在高盐杂交缓冲液中极低的聚集性
• Well suited for nucleic acid applications with extreme demands
•非常适合有极端需求的核酸应用
• Low nonspecific binding of small and negatively charged proteins
•带负电荷的小蛋白质的非特异性结合低
•低
• Production follows a validated process in compliance with cGMP for medical devices
•生产遵循符合cGMP的医疗器械验证流程
• Well suited for automated protocols
•非常适合自动化协议
About Dynabeads® M-270 Streptavidin
关于Dynabeads®M-270 Streptavidin
These uniform and superparamagnetic beads are 2.8 µm in diameter, with a monolayer, not a multilayer, of recombinant streptavidin covalently coupled to the surface. This leaves the vast majority of the biotin binding sites sterically available for binding, not only of free biotin, but also for binding of biotinylated ligands/targets. They are hydrophilic, negatively charged and show rapid liquid-phase reaction kinetics. Their specific and defined surface allow for efficient capture, separation, and downstream handling. The streptavidin monolayer ensures negligible leakage, and the lack of excess adsorbed streptavidin ensures batch consistency and reproducibility of your results. Applications
这些均匀的超顺磁性磁珠直径为2.8µm，具有共价耦合到表面的重组链霉亲和素单层而非多层。这使得绝大多数生物素结合位点在空间上可用于结合，不仅是游离生物素，而且也可用于生物素化配体/靶点的结合。它们是亲水的，带负电，表现出快速的液相反应动力学。其特定和明确的表面允许有效的捕捉、分离和下游处理。链霉亲和素单层膜可确保可忽略的泄漏，而缺乏过量吸附的链霉亲和素可确保批量一致性和结果的重现性。
应用 Over the past 15 years, streptavidin-coupled Dynabeads® have been used and cited for a very wide variety of applications. Examples include direct/indirect isolation and downstream handling of nucleic acids, proteins/peptides, and other target molecules. Ideal for sequence-specific DNA/RNA capture in nucleic acid–based diagnostics, specifically with samples with a high chaotropic salt concentration, immunoassays involving small biotinylated antigens, and applications that are not compatible with BSA (these beads are not blocked with BSA). Easily adaptated to automated processes. Dynabeads® are used on more than 25,000 routine IVD instruments worldwide. The product holds high standards with respect to reproducibility (both within and between batches), and automation ability, and drives reliability for your results.
在过去的15年中，链霉亲和素偶联的Dynabeads®已被广泛应用于各种应用。例如直接/间接分离和下游处理核酸、蛋白质/肽和其他靶分子。非常适合于核酸诊断中的序列特异性DNA/RNA捕获，特别是具有高朝向性盐浓度的样本，涉及小生物素化抗原的免疫分析，以及与BSA不兼容的应用（这些微珠不会被BSA阻断）。易于适应自动化流程。Dynabeads®在全球25000多个常规IVD仪器上使用。该产品在再现性（批内和批间）和自动化能力方面具有高标准，并提高了结果的可靠性。
Binding capacity
结合力
The size of the molecule and the biotinylation procedure will affect the binding capacity. The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules. There are two or three biotin binding sites available for each streptavidin molecule on the surface of the bead after immobilization. One mg of Dynabeads® M-270 Streptavidin typically binds:
分子的大小和生物素化过程都会影响结合能力。其容量还取决于珠粒与分子之间以及分子间的空间位阻有效性和电荷相互作用。每个链霉亲和素分子在固定化后的珠表面有两到三个生物素结合位点。一毫克Dynabeads®M-270链霉亲和素通常结合：
• >950 pmoles free biotin
•>950 pmoles游离生物素
• ~200 pmol biotinylated peptides
•~200 pmol生物素化肽
• ~10 µg biotinylated IgG
•~10微克生物素化IgG
• ~10 µg ds-DNA
•~10微克ds DNA
• ~200 pmol ss-oligonucleotides
•~200 pmol ss寡核苷酸
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