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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
零下80~20°C
- 保质期:
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名:
CREBtide
- 库存:
10
- 供应商:
TargetMol
- CAS号:
149155-45-3
- 规格:
1 mg/5 mg/10 mg
| 规格: | 1 mg | 产品价格: | ¥578.0 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | ¥1820.0 |
| 规格: | 10 mg | 产品价格: | ¥2910.0 |
Product Introduction
Bioactivity
英文名:CREBtide
描述:CREBtide is a synthetic substrate for PKA (Km=3.9 µM), which is based on the phosphorylation sequence in d-CREB (cAMP response element binding protein).

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文献和实验Preparation of Affinity Column制备亲和层析柱【UCSF】
1.Add 222.2μl 1M MOPS,pH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final concentration). 2.Read OD205,OD280 (using 0.1M MOPS buffer as blank) 3.Affigel-10 is stored frozen at 4.Filter 1-2ml on nylon (0.22uM poresize),using Buchner funnel
Preparation of Affinity Column
Preparation of Affinity Column SEK 5/3/95 1.Add 222.2μl 1M MOPS,pH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final concentration). 2.Read OD205,OD280 (using 0.1M MOPS buffer as blank) 3.Affigel-10 is stored frozen at 4.Filter 1-2
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