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- TargetMol
- 美国
- T5300
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
零下80~20°C
- 保质期：
Powder: -20°C for 3 years In solvent: -80°C for 2 years
- 英文名：
Ammonium formate
- 库存：
10
- 供应商：
TargetMol
- CAS号：
540-69-2
- 规格：
10 g
Product Introduction
Bioactivity
英文名:Ammonium formate
描述:Ammonium formate (Formic acid ammonium salt) is the simplest carboxylic acid. Formate is an intermediate in normal metabolism. It is responsible for both metabolic acidosis and disrupting mitochondrial electron transport and energy production by inhibiting cytochrome oxidase activity, the terminal electron acceptor of the electron transport chain. Cell death from cytochrome oxidase inhibition by formate is believed to result partly from depletion of ATP, reducing energy concentrations so that essential cell functions cannot be maintained. Furthermore, inhibition of cytochrome oxidase by formate may also cause cell death by increased production of cytotoxic reactive oxygen species (ROS) secondary to the blockade of the electron transport chain.
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文献和实验催化甲酸脱氢生成二氧化碳反应的酶。存在于高等植物的酶（EC1． 2． 1． 2）。以 NAD 为电子受体， HCOOH NAD → CO2 NADH H 。可为氰化物或叠氮化物所抑制。大肠杆菌等细菌的酶（ EC1． 2． 2． 1）以细胞色素 b1或泛醌（ UQ8 ）为受体，由于受含于颗粒部分的不溶性酶或氰化物等的抑制，认为是一种金属酶。此酶与甲酸的氧化酶或氢化酶偶联，与甲酸分解成氢和二氧化碳有关系。在动物的肝脏里也存在着必需 NAD 和 AMP的酶。
Ammonium Sulfate Binding Assay for Cells
1. Resuspend cells (vegetative or developed) in phosphate buffer at 1 x 10 8 /ml . 2. Add 50 ul cells to 50 ul 20 nM 3H-cAMP in 5-10 mM DTT (for background binding, add same amount of hot cAMP plus 10 uM cold cAMP) in 1.5 ml eppendorf tube
Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration
Antibodies can be purified by a variety of methods based on their unique physical and chemical properties such as size, solubility, charge, hydrophobicity and binding affinity. This chapter focuses on ammonium sulfate precipitation
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