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- 文献和实验
- 技术资料
- 保存条件：
2-8 °C
- 保质期：
24
- 英文名：
StrepMAB-Classic, HRP conjugate
- 库存：
999+
- 供应商：
IBA Lifesciences
- 规格：
75 µl
StrepMAB-Classic is a highly selective, murine monoclonal antibody for detection of Strep-tag®II and Twin-Strep-tag® fusion proteins in applications like western blots, ELISA, immunohistochemistry, and immunocytochemistry. It is available coupled to fluorescent dyes (DY-488, DY-549, DY-649) or to horseradish peroxidase (HRP). StrepMAB-Classic HRP is suitable for chromogenic and chemiluminescent reactions.
The absorption/emission maxima of the fluorescent dyes are:
• DY-488: 493/518 nm
• DY-549: 550-560/575 nm
• DY-649: 655/676 nm
Specifications
| Amount: | StrepMAB-Classic HRP: 75 µl, StrepMAB-Classic DY-488/DY-549/DY-649: 100 µl |
|---|---|
| Clonality: | Monoclonal |
| Concentration: | StrepMAB-Classic HRP: 1.5 mg/ml, StrepMAB-Classic DY-488/DY-549/DY-649: 0.5 mg/ml |
| Conjugate: | HRP, DY-488, DY-549, DY-649 |
| Form: | Suspension in PBS |
| Possible Application: | ELISA, western blot, Immunofluorescence |
| Specificity: | Strep-tag®II and Twin-Strep-tag® |
| Subclass: | IgG1 |
| Species: | Mouse |
Shipping information
| Stability: | 24 months after shipping |
|---|---|
| Shipping: | Room temperature |
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文献和实验【2】Guo H, de Vries E, McBride R, et al. Highly pathogenic influenza A (H5Nx) viruses with altered H5 receptor-binding specificity[J]. Emerging infectious diseases, 2017, 23(2): 220.
【3】Piuri M, Rondón L, Urdániz E, et al. Generation of affinity-tagged fluoromycobacteriophages by mixed assembly of phage capsids[J]. Applied and environmental microbiology, 2013, 79(18): 5608-5615.
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8oC. Dialyze against 3 changed of PBS, changing every 2 hours, 100X the conjugate
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
Direct Attachment of Conjugate Groups to the 5′ Terminus of Oligodeoxyribonucleotides
protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protocols are given for acridine? and psoralen?conjugated oligonucleotides, and include attachment of the linker, preparation
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