手机验证
Chromatin Immunoprecipitation
Rabbit
大量
0
Human;Mouse;Mammals
2
25 assays
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from UV treated (50 J/m2, 6 hrs.) U2OS cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or Anti-Acetyl-Histone H3 (Lys9) antibody (Cat. #CS200583) and the Magna ChIP A (Cat. #17-610) Kit. Successful immunoprecipitation of acetyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using control ChIP Primers p21, (Cat. #CS200575) flanking the human p21(WAF1/CIP1/CDKN1A) promoter (Figure 1).
Please refer to the EZ-Magna A ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated or UV treated (50 J/m2, 6 hrs.) U2OS cells (3 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or Anti-Acetyl-Histone H3 (Lys9) antibody (Cat. #CS200583) and the Magna ChIP A (Cat. #17-610) Kit. Successful immunoprecipitation of acetyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using control ChIP Primers p21, (Cat. #CS200575) flanking the human p21(WAF1/CIP1/CDKN1A) promoter (Figure 2). Data is presented as fold enrichment of normalized percent input of each IP sample relative to input treated or untreated chromatin.
Please refer to the EZ-Magna A ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.
Western Blot Analysis:
Representative blot from a previous lot. Acid-extracted proteins from normal HeLa cells (Lane 1) and HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Acetyl Histone H3 (Lys9) (1 μg/mL). Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Figure 3).
Arrow indicates acetylated Histone H3 (Lys9) (~17 kDa). Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H3 (Lys9) (Lane 2).
Species Reactivity | Key Applications | Host | Format | Antibody Type |
---|---|---|---|---|
H, M, Ma | Chromatin Immunoprecipitation (ChIP), WB | Rabbit | null | Polyclonal Antibody |
Histone H3Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
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