- 询价
- 17-658
- 2025年12月13日
- Chromatin Immunoprecipitation
- Rabbit
- Human;Mouse;Mammals
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Chromatin Immunoprecipitation
- 宿主：
Rabbit
- 库存：
大量
- 抗原来源：
0
- 适应物种：
Human;Mouse;Mammals
- 是否单克隆：
2
- 规格：
25 assays
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from UV treated (50 J/m2, 6 hrs.) U2OS cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or Anti-Acetyl-Histone H3 (Lys9) antibody (Cat. #CS200583) and the Magna ChIP A (Cat. #17-610) Kit. Successful immunoprecipitation of acetyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using control ChIP Primers p21, (Cat. #CS200575) flanking the human p21(WAF1/CIP1/CDKN1A) promoter (Figure 1).
Please refer to the EZ-Magna A ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from untreated or UV treated (50 J/m2, 6 hrs.) U2OS cells (3 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or Anti-Acetyl-Histone H3 (Lys9) antibody (Cat. #CS200583) and the Magna ChIP A (Cat. #17-610) Kit. Successful immunoprecipitation of acetyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using control ChIP Primers p21, (Cat. #CS200575) flanking the human p21(WAF1/CIP1/CDKN1A) promoter (Figure 2). Data is presented as fold enrichment of normalized percent input of each IP sample relative to input treated or untreated chromatin.
Please refer to the EZ-Magna A ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.
Western Blot Analysis:
Representative blot from a previous lot. Acid-extracted proteins from normal HeLa cells (Lane 1) and HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Acetyl Histone H3 (Lys9) (1 μg/mL). Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Figure 3).
Arrow indicates acetylated Histone H3 (Lys9) (~17 kDa). Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H3 (Lys9) (Lane 2).
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Ma | Chromatin Immunoprecipitation (ChIP), WB | Rabbit | null | Polyclonal Antibody |
- Chromatin Immunoprecipitation (ChIP)
- Western Blotting
Sonicated chromatin prepared from untreated or UV treated (50 J/m2, 6 hrs.) U2OS cells (3 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 5 μg of either a normal rabbit IgG or Anti-Acetyl-Histone H3 (Lys9) antibody and the Magna ChIP A (Cat. #17-610) Kit. Successful immunoprecipitation of acetyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using control ChIP Primers p21 flanking the human p21(WAF1/CIP1/CDKN1A) promoter (Please see figures). Data is presented as fold enrichment of normalized percent input of each IP sample relative to input treated or untreated chromatin.
Please refer to the EZ-Magna A ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.
Western Blot Analysis:
Acid-extracted proteins from normal HeLa cells (Lane 1) and HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-Acetyl Histone H3 (Lys9) (1 μg/mL). Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
- Human
- Mouse
- Mammals
Normal Rabbit IgG. One vial containing 125 ug purified Rabbit IgG in 125 uL storage buffer containing 0.05% sodium azide.
ChIP Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter.
FOR: GTG GCT CTG ATT GGC TTT CTG
REV: CTG AAA ACA GGC AGC CCA AG
- H3F3A
- H3F3B
- H3F3
Sonicated chromatin prepared from UV treated (50 J/m2, 6 hrs.) U2OS cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 5 μg of either a normal rabbit IgG or Anti-Acetyl-Histone H3 (Lys9) antibody and the Magna ChIP A (Cat. #17- 610) Kit. Successful immunoprecipitation of acetyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using control ChIP Primers p21 flanking the human p21(WAF1/CIP1/CDKN1A) promoter (Please see figures).
Please refer to the EZ-Magna A ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17- 371) protocol for experimental details.
The ChIPAb+ Acetyl-Histone H3 (Lys9) set includes the anti-acetyl-histone H3 (Lys9) antibody, a negative control antibody (purified Rabbit IgG), and qPCR primers flanking an Sp1 binding site in the human p21 (WAF1/CIP1/CDKN1A) promoter, amplifying a 105 base pair PCR product. The acetyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of acetyl-histone H3 (Lys9) associated chromatin.
Product Family Information
Histone H3Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
The acetyl-histone H3 (Lys9) purified antibody is made against a peptide (acetylated at Lys9) corresponding to amino acids 1-12 of histone H3. 详细描述见链接：http://www.millipore.com/catalogue/item/17-658
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文献和实验3.75ml 4M NaCl 3ml 10% Triton X (Tx-100) 300µl 10% NAD 21.45ml H20 ChIP wash buffer 30ml final volume: 150µl 2M Tris 1.7ml 4.4M LiCl 60µl 0.5M EDTA 1.5ml 10% NAD 1.5ml 10% NP-40 25.09ml
Evaluation of Histone-Modifying Enzymes in Stem Cell Populations
the bioactivity from both affinity-purified complexes and reconstituted complexes and directly in the cell. These assays have allowed us to define a set of factors that regulate the KDM5 family of histone demethylases.
," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
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