The GenoStat CHO K-1 Stable Cell Line Kit contains everything a researcher needs to facilitate the expression of exogenous genes when utilizing the GenoStatTM Inducible Expression System (cat. no. 17-428).
The CHO K-1 Stable Cell Line has stably integrated the pGS-1 Receptor plasmid (cat. no. 21-208), a key component of the inducible expression system, into its genome. These CHO K-1 cells will facilitate the inducible expression of exogenous genes when used in conjunction with the pGS-2 Expressor plasmid. The end user can simply subclone their gene of interest into the pGS-2 plasmid and then transfect it into these stable CHO K-1 cells. Following transfection, GenoStatTM Ligand (cat. no. 20-333 or 20-382) is added to the culture media to turn 'on' expression of the subcloned gene. The pGS-Luciferase Plasmid is provided as an expression control that can be utilized in place of the pGS-2 plasmid to optimize expression conditions. Detailed instructions for the use of each of these kit components are provided in their certificates of analysis.
Key Applications:
RNAi/siRNA/Gene Knockdown
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
GenoStat™ CHO K-1 Stable Cell Line (Cat.# 20-387)
GenoStat™ pGS-2 Expressor Plasmid (Cat.# 21-209)
GenoStat™ pGS-Luciferase Plasmid (Cat.# 21-210)
GenoStatTM Ligand (50 μL of 5 mM stock)
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
CHO cell line with stably integrated pGS-1 Receptor plasmid (cat. # 21-208).