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- 详细信息
- 文献和实验
- 技术资料
- 库存:
18
- 英文名:
9N (exo-) DNA Polymerase
- 保质期:
1年
- 供应商:
上海泽叶生物科技有限公司
- 保存条件:
-20
- 规格:
100U/1000U
| 9N (exo-) DNA Polymerase 是9N DNA聚合酶中的突变体,具有较强的掺入修饰底物的能力,如:ddNTP、rNTP 和acyNTP,因此其适用于核糖核酸置换法DNA测序、ddNTP或acyNTP的链终止法测序或SNP分析。该酶来源于E. coli菌株,此菌株含有从Thermococcus species 9°N-7 中克隆的经过基因工程改造的9°N (D141A/E143A/A485L) DNA聚合酶基因。 | ||||||||
主要特征和应用 |
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组分 |
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单位定义 |
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| 一个活力单位即在在74°C条件下,30分钟内催化10 nmol dNTP的掺入反应成为酸不溶性物质所需的酶量。 | ||||||||
储存 |
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| -20℃可保存3年。 | ||||||||
使用方法 |
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文献和实验We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template
Exo Ovo Culture of Avian Embryos
Exo ovo culture of avian embryos is a technique for long-term culturing of embryos outside of their own shell and shell membranes. The problem of how to gain access to the avian embryo while allowing it to grow normally has been the subject
The Polymerase Chain Reaction (PCR)
. Did He Really Invent PCR? • The basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khorana in 1971:– Kleppe et al. (1971) J. Mol. Biol. 56, 341-346. • Progress was limited by primer synthesis and polymerase
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