PneumaCult™-ALI

PneumaCult™-ALI培养基用于在气-液界面上培养人大气道上皮细胞。在PneumaCult™-ALI培养基中培养的气道上皮细胞经过彻底的粘膜纤毛分化，形成假复层上皮，其表现出与人体内气道相似的形态和功能特征。PneumaCult™-ALI培养基和PneumaCult™-Ex Plus培养基共同构成了完整的，构建人大气道体外模型的培养系统，是基础呼吸研究，毒性研究和药物开发的重要工具。

有研究指出，PneumaCult™-ALI的气-液界面方法来生成分化的气道上皮粘膜纤毛培养物。文章指出使用气-液界面培养建立的研究模型可以模仿体内气道表皮细胞对II型细胞因子以及IL-13做出生理学反应，具备高度的呼吸系统疾病研究价值。

此外，PneumaCult™-ALI还可用于进行婴幼儿呼吸道表皮细胞的建立。有研究指出使用PneumaCult™-ALI对来自婴儿的原代上皮细胞进行气-液界面培养，经过后续检测，细胞表现出呼吸道上皮细胞的四种细胞表型。为研究婴幼儿呼吸道发育以及相关研究提供了重要的实验模型。

PneumaCult™-ALI Medium (Catalog #05001) is a serum- and BPE-free medium for the culture of human airway epithelial cells at the air-liquid interface (ALI). Airway epithelial cells cultured in PneumaCult™-ALI Medium undergo extensive mucociliary differentiation to form a pseudostratified epithelium that exhibits morphological and functional characteristics similar to those of the human airway in vivo. PneumaCult™-ALI Medium is also available in a kit that includes 12 mm Transwell® inserts (Catalog #05021) or 6.5 mm Transwell® inserts (Catalog #05022).

Together, PneumaCult™-ALI Medium and PneumaCult™-Ex Plus Medium (Catalog #05040) constitute a fully integrated BPE-free culture system for in vitro human airway modeling. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.

Advantages:
• HBECs cultured with PneumaCult™-ALI undergo extensive mucociliary differentiation to form a pseudostratified epithelium that closely resembles the human airway
• PneumaCult™-ALI is serum-free and BPE-free to minimize variability

Components:

• PneumaCult™-ALI Basal Medium, 450 mL

• PneumaCult™-ALI 10X Supplement, 50 mL

• PneumaCult™-ALI Maintenance Supplement (100X), 5 x 1 mL

Cell Type:
呼吸道细胞

Species:
人

Formulation:
无血清