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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PAK1 Antibody抗体
- 抗体英文名:
PAK1 Antibody
- 靶点:
PAK1
- 应用范围:
ELISA, IHC, WB
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb629637
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
liquid
- 亚型:
IgG
- 免疫原:
p21 protein (Cdc42/Rac)-activated kinase 1
- 规格:
50 ug
别名:Alpha PAK, p21 activated kinase 1, p65 PAK, PAK 1, PAK1, PAKalpha
免疫原:p21 protein (Cdc42/Rac)-activated kinase 1
分子量:62 kDa
应用稀释比例:WB: 1:500 - 1:2000; IHC: 1:50 - 1:200
纯度:≥95% as determined by SDS-PAGE
纯化:Immunogen affinity purified
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:Q13153
Note:For research use only.


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文献和实验Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases
with a fluorescent protein produces a probe that is recruited to compartments where GTPases are active. We describe an example of a recruitment assay using the GBD of PAK1 to monitor Rac1 activity and explain how the assay can be expanded
(GST)或者是 GST fusion protein包含了Cdc42/Rac binding domain (CRIB) of PAK1 immobilized on glutathione–Sepharose 4B beads. The beads 用 lysis buffer清洗三次, bound GTP-Rac1 采用Rac1抗体进行免疫印渍分析 就我目前的了解来说,Rac和Cdc42都是可以采取这样的方式来检测其活性的.
14. MKK: MAP2K1, MAP2K2, MAP2K3, MAP2K4, MAP2K5, MAP2K6, MAP2K7. MKKK: ARAF1, BRAF, DLK1, MAP3K1, MAP3K2, MAP3K3, MAP3K4, MAP3K5, MAP3K7, MAP3K11, MAP3K14, MAP4K1, MAP4K3, MOS, MST1, PAK1, PAK3, RAF1. NFAT Family Members: NFATC1, NFATC2, NFATC3
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