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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见包装
- 保质期：
见包装
- 英文名：
Creatine Kinase MM (CK-MM) Antigen >95%
- 库存：
999
- 供应商：
博奥派克
- 规格：
1 mg
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文献和实验Serum Creatine Kinase in Progressive Muscular Dystrophies
Several enzymes originating in muscle show increased activity in the serum of patients affected with different forms of muscular dystrophy (MD). These enzymes include creatine kinase (CK), aldolase, transaminase, and pyruvate kinase (PK
by vortexing every 10 min. Stop reaction by adding 15 µL of 4x sample buffer. Heat on 95°C block for 5 min. (Can be stored at 4°C overnight) Spin down beads and load supernatant on a 12% SDS acrylamide gel. Run off the dye front (175 volts x
(all concentrations are final) PBST - Store in fridge 150 mM NaCl 16 mM Na2HPO4 4mM NaH2PO4 0.1 % Triton X 100 HYPOTONIC LYSIS SOLUTION - Store in the fridge 0.1 mM PMSF 100 µM Na3VO4 20 µg/ml leupeptin 50 mM NaF 1 mM benzamidine KINASE
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