Core histones were purified from HeLa cells nuclei. After digestion of the nuclei with micrococcal nuclease, the histones are isolated over a hydroxyapatite column. Purity ≥ 90% by SDS PAGE and Coomassie blue staining.
Quality Assurance:
Chromatin Assembly Assay: Activity is routinely tested with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410). ~2.0 µg core histones have been successfully used for 1 chromatin assembly reaction.
Source:
Core histones purified from HeLa cells nuclei.
Presentation:
50 μg of core histones in 50 μl of 10 mM HEPES pH 7.5, 1 mM EDTA, 1 mM beta-mercaptoethanol, 0.1 M PMSF, 20% Glycerol. Frozen solution.
Storage Conditions:
Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.