SMURF2 Antibody 抗体,orb570354,Biorbyt

SMURF2 Antibody 抗体,orb570354,B

iorbyt
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  • ¥3484 - 6760
  • Biorbyt已认证
  • orb570354
  • 英国
  • 2025年07月08日
  • ELISA, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 技术资料
    • 抗体名

      SMURF2 Antibody 抗体

    • 抗体英文名

      SMURF2 Antibody

    • 浓度

      Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    • 应用范围

      ELISA, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb570354

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      POLYCLONAL

    • 保存条件

      Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

    • 形态

      Lyophilized

    • 亚型

      Rabbit IgG

    • 免疫原

      E.coli-derived human SMURF 2/SMURF2 recombinant protein (Position: W303-Q387).

    • 规格

      10 ug/100 ug

    规格:10 ug产品价格:¥3484.0
    规格:100 ug产品价格:¥6760.0
    SMURF2 Antibody
    产品名:SMURF2 Antibody
    货号:orb570354
    产品中文名:SMURF2 Antibody 抗体
    产品详情链接:https://www.biorbyt.com/smurf2-antibody-orb570354.html

    SMURF2 Antibody 抗体,orb570354,B
     
    Western blot analysis of SMURF2 using anti-SMURF2 antibody (orb570354). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testicular issue lysates, Lane 2: rat pancreas issue lysates, Lane 3: rat stomach issue lysates, Lane 4: mouse testicular issue lysates, Lane 5: mouse pancreas issue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (Catalog # orb570354) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86KD. The expected band size for SMURF2 is at 86KD.

    SMURF2 Antibody 抗体,orb570354,B
     
    Western blot analysis of SMURF2 using anti-SMURF2 antibody (orb570354). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (Catalog # orb570354) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86KD. The expected band size for SMURF2 is at 86KD.

    产品主推四大类:

    一. 抗体(Antibody):79万种的一抗。可应用于Western blot,IP,IHC,IF,FACS,ELISA等实验。
    二. 蛋白(Protien):13万种的高纯度蛋白。
    三. elisa试剂盒(Elisa kit):可以提供超过9万种的ELISA试剂盒,包括人源,大鼠源,小鼠源等。
    四. 小分子(Small Molecules):超过12万种的生物活性小分子,主要包括:拮抗剂、抑制剂、抗生素、天然产物。

    所有Biorbyt品牌的抗体,蛋白,Elisa试剂盒,小分子等产品,国际航空低温运输,货期短,多数产品货期在1~2周,快捷便利。
    我们为研究者们提供免费的、专业的、售前售中售后服务,为您的实验保驾护航。

    更多详情请联系我们:027-87663101、15827569716。

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