Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
应用范围:
FC, ICC, IF, IHC, IP, WB
宿主:
Rabbit
适应物种:
Human, Mouse
保质期:
6-12个月
抗原来源:
详询
目录编号:
orb548142
级别:
科研
库存:
99
供应商:
Biorbyt
标记物:
Unconjugated
克隆性:
MONOCLONAL
保存条件:
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
形态:
Liquid
亚型:
Rabbit IgG
免疫原:
A synthesized peptide derived from human CD97 Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
Western blot analysis of CD97 expression in U937 cell lysate using anti-CD97 antibody (orb548142). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD97 antigen affinity purified polyclonal antibody (Catalog # orb548142) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for CD97.