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- 详细信息
- 技术资料
- 应用范围:
Western Blotting;Immunocytochemistry
- 宿主:
Rabbit
- 抗原来源:
0
- 库存:
大量
- 适应物种:
Human
- 是否单克隆:
2
- 规格:
100 μL
Product Images
TPA treated lysates from CHO cells transfected with human IRS1 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IC, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
anti-phospho-IRS1 (Ser616)
Alternate Names:
IRS-1, Insulin Receptor Substrate 1
Specificity:
Human IRS1 (Insulin Receptor Substrate 1) phosphorylated on Serine residue 616. Sequence 100% homologous with mouse, rat, and pig.
Key Applications:
- Immunocytochemistry
- Western Blotting
Application Notes:
Immunoblot Analysis: 0.5 μg/mL dilutions of this antibody detected phospho-IRS1 (Ser616) in RIPA lysates from TPA treated CHO cells transfected with human IRS1.
Species Reactivity:
Human
Species Reactivity Note:
Tested on human. Predicted to cross-react with mouse and rat based on 100% sequence homology, but have not been tested.
Isotype:
IgG
Immunogen:
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-IRS-1 (Ser616)
Modifications:
Phosphorylation
Molecular Weight:
165 kDa
Presentation:
100 μL of affinity purified rabbit polyclonal IgG in PBS (without Mg2+ and Ca2+ ), pH 7.3, containing 1.0 mg/mL BSA (IgG, protease free), 0.05% sodium azide and 50% glycerol.
Storage Conditions:
1 year at -20°C.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- INSR
- CD220
- HHF5
- IR
Quality Assurance:
Evaluated by immunoblot.
Antibody Category:
Signaling
Antibody Sub-Category:
Insulin/Energy Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Rabbit
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-IRS-1 (Ser616) 详细描述见链接:http://www.millipore.com/catalogue/item/07-843
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anti-phospho-IRS1 (Ser616); 100 μL
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