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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Western Blotting;Immunocytochemistry
- 宿主:
Rabbit
- 抗原来源:
0
- 库存:
大量
- 适应物种:
Human
- 是否单克隆:
2
- 规格:
100 μL
Product Images
TPA treated lysates from CHO cells transfected with human IRS1 were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-p...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IC, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
- Immunocytochemistry
- Western Blotting
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.
- INSR
- CD220
- HHF5
- IR
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-IRS-1 (Ser616) 详细描述见链接:http://www.millipore.com/catalogue/item/07-843
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文献和实验separating buffer with 13.0 mL of water, 10.5 mL of acrylamide/bis solution, 100 μL of ammonium persulfate solution, and 20 μL of TEMED. Pour the separating gel,and overlay with water-saturated isobutanol. 3. Pour off the isobutanol and rinse
:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
amounts of lysate(usually 0.1–10 μL in a total volume of 100 μL of water) to1 mL of Coomassie (Bradford) reagent, vortex, and incubateat room temperature for 10 min. Vortex the solutions again,then measure the absorbance using a spectrophotometer atl max
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