- 询价
- 07-841
- 2025年07月14日
- Western Blotting
- Rabbit
- Human;Mouse
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- 详细信息
- 询价记录
- 技术资料
- 应用范围：
Western Blotting
- 宿主：
Rabbit
- 库存：
大量
- 抗原来源：
0
- 适应物种：
Human;Mouse
- 是否单克隆：
2
- 规格：
100 μL
Product Images
Untreated (Lane 1) or insulin treated (Lanes 2, 3, and 4) lysates from CHO cells transfected with IR were resolved by electrophoresis, transferred to ...
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-phospho-IR/IGF1R (Tyr1158/Tyr1162/Tyr1163)
Alternate Names:
CD220, Insulin Receptor / CD221, Insulin-like Growth Factor 1 Receptor
Specificity:
Human and mouse phospho-Insulin Receptor (IR) Tyrosine 1158/1162/1163 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131/1135/1136. Rat reactivity is expected based on 100% sequence homology.
Key Applications:
Western Blotting
Application Notes:
Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-IR (Tyr1158/Tyr1162/Tyr1163) in RIPA lysate of untreated and insulin treated CHO cells transfected with IR.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Predicted to cross-react with rat based on 100% sequence homology, but have not been tested.
Isotype:
IgG
Immunogen:
Peptide corresponding to the amino acid region encompassing the human, mouse, and rat phospho-Insulin Receptor (IR) Tyrosine 1158, 1162, and 1163 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131, 1135, and 1136.
Modifications:
Phosphorylation
Molecular Weight:
155 kDa
Presentation:
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+ ), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
Storage Conditions:
Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Gene Symbol:
- INSR
- CD220
- HHF5
- IR
Quality Assurance:
Routinely evaluated by immunoblot.
Antibody Category:
Signaling
Antibody Sub-Category:
Insulin/Energy Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Rabbit
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Peptide corresponding to the amino acid region encompassing the human, mouse, and rat phospho-Insulin Receptor (IR) Tyrosine 1158, 1162, and 1163 and Insulin-like Growth Factor 1 Receptor (IGF1R) Tyr 1131, 1135, and 1136. 详细描述见链接：http://www.millipore.com/catalogue/item/07-841
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anti-phospho-IR/IGF1R (Tyr1158/Tyr1162/Tyr1163); 100 μL
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