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Anti-CTCF; 200 μL

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  • 询价
  • 07-729
  • 2025年07月16日
  • Chromatin Immunoprecipitation
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围

      Chromatin Immunoprecipitation

    • 宿主

      Rabbit

    • 抗原来源

      0

    • 库存

      大量

    • 适应物种

      Human

    • 是否单克隆

      2

    • 规格

      200 μL

    Product Images

    WB: HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CTCF (1:2000 dilution). Arrow indicates...

    Species Reactivity Key Applications Host Format Antibody Type
    H, R, Ca, M, Pm Chromatin Immunoprecipitation (ChIP), WB Rabbit Serum Polyclonal Antibody
    Description:
    Anti-CTCF
    Background Information:
    CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma.
    Alternate Names:
    • 11 zinc finger transcriptional repressor
    • 11-zinc finger protein
    • CCCTC-binding factor
    • CCCTC-binding factor (zinc finger protein)
    • CTCFL paralog
    • transcriptional repressor CTCF
    Specificity:
    Recognizes CTCF at a.a. 659-675.
    Key Applications:
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    Application Notes:
    Chromatin Immunoprecipitation:
    An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.
    Species Reactivity:
    • Human
    • Rat
    • Canine
    • Mouse
    • Primate
    Species Reactivity Note:
    Human. Predicted to cross-react with mouse, rat, dog and primates based on sequence homology.
    Immunogen:
    KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes.
    Molecular Weight:
    Approx. 120 kDa
    Control:
    HeLa nuclear extract.

    Included Positive Antigen Control:
    Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels.
    Presentation:
    Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol.
    Storage Conditions:
    Stable for 1 year at -20°C from date of receipt.
    Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
    Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
    Gene Symbol:
    CTCF
    Quality Assurance:
    Routinely evaluated by Western Blot.

    Western Blot Analysis:
    A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown).
    Antibody Category:
    Epigenetics & Nuclear Function
    Antibody Sub-Category:
    Chromatin Biology
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Upstate (Millipore)
    Concentration:
    Variable
    Antibody Type:
    Polyclonal Antibody
    Format:
    Serum
    Host:
    Rabbit

    更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com

    KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes. 详细描述见链接:http://www.millipore.com/catalogue/item/07-729

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