相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验of interest. To prevent antibody cross-detection, make sure that the species origin of this antibody differs from that of the anti-DIG antibody used to detect the FISH probe. 3. Select a fluorochrome-conjugated secondary antibody directed against the species
注:此后步骤应注意避光操作。 1.2 滴加一抗 将4 μl Anti-DIG monoclonal antibody 和0.5 μl Texas-red-Avidin 稀释于100 μl 抗体稀释液1中,混匀;每张玻片滴加25 μl 于杂交区域,盖上22mm×22mm盖玻片,于37℃湿盒中孵育1小时;室温下4×SSC/0.1 % Tween-20中振荡洗涤5min×2缸。 1.3 滴加二抗
Single and Double FISH protocols for Drosophila
. 2. PBT solution: 1XPBS, 0.1% Tween-20. 3. PBTB solution: 1XPBS, 0.1% Tween-20 and 0.5% milk powder. 4. Mouse monoclonal anti-fluorescein antibody (IgG: 1/2000 dilution of a 0.1 mg/ml stock solution [see notes 2,3]; Boehringer Mannheim, Laval
技术资料暂无技术资料 索取技术资料











