Myeloperoxidase Peroxidation F

Cayman的MPO过氧化荧光测定法提供了一种方便的荧光法，用于检测粗细胞裂解物和纯化酶制剂中的MPO过氧化物酶活性。该测定法利用MPO的过氧化物酶成分，其中天然酶(MPO)经过单次双电子氧化为化合物I(MPO-I)，然后由化合物II(MPO-II)连续两次单电子还原回到天然酶。7过氧化氢与ADHP(10-乙酰基-3,7-二羟基吩恶嗪)之间的反应产生高荧光化合物试卤灵。试卤灵荧光可以轻松分析，激发波长为530-540nm，发射波长为585-595nm。该试剂盒包含MPO特异性抑制剂，用于区分MPO活性与MPO非依赖性荧光。Cayman's MPO Peroxidation Fluorometric Assay provides a convenient fluorescence-based method for detecting the MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO, where a single two electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one electron reductions back to native enzyme by compound II (MPO-II).7 The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence can be easily analyzed with an excitation wavelength of 530-540 nm and emission wavelength of 585-595 nm. The kit includes a MPO-specific inhibitor for distinguishing between MPO activity from MPO-independent fluorescence.分子式分子量