NAD/NADH Cell-Based Assay Kit

烟酰胺腺嘌呤二核苷酸(NAD)以氧化形式NAD+和还原形式NADH存在。NAD是细胞中的主要游离形式，通过调节细胞氧化还原状态和控制信号传导和转录事件发挥作用，使其成为研究正常细胞功能时的重要辅助因子。Cayman的NAD/NADH细胞分析试剂盒提供了一种比色法，用于测量培养细胞中的细胞内NAD+和NADH。在该分析中，在乙醇氧化为乙醛的过程中，细胞样本中的NAD+被醇脱氢酶还原为NADH。然后，样本中新形成的和现有的NADH被氧化，导致四唑盐底物(WST-1)还原为高色度的甲臜，其吸收波长为450nm。产生的甲臜的量与细胞裂解物中的总NAD的量成正比，可以作为细胞总NAD浓度的指标。Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form, NAD+, as well as a reduced form, NADH. NAD, the main free form in cells, functions in modulating cellular redox status and by controlling signaling and transcriptional events, making it an important cofactor when investigating normal cellular function. Cayman's NAD/NADH Cell-Based Assay Kit provides a colorimetric ethod for measuring intracellular NAD+and NADH in cultured cells. In this assay, NAD+ found in cell samples is reduced to NADH by alcohol dehydrogenase during the oxidation of ethanol to acetaldehyde. The newly formed and the existing NADH found in the samples is then oxidized resulting in the reduction of a tetrazolium salt substrate (WST-1) to a highly-colored formazan which absorbs at 450 nm. The amount of formazan produced is proportional to the amount of total NAD in the cell lysate and can be used as an indicator of the total cellular NAD concentration.分子式分子量