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- 2025年07月16日
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文献和实验Picolinyl esters for the structural determination of fatty acids by GC/MS
The position of unsaturation, chain branching, and other structural features of fatty acids are not often apparent from the mass spectra of common derivatives such as methyl esters because of factors such as charge location at the carboxy
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
Experimental protocol 1. Run a blank with 1 µl of hexane. 2. Run 1 µl of fatty acid methyl ester standard to calibrate the column. Name % Rt(example) Rt observed 14:0ME 1 4.9 min 16:0ME
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
of fatty acid methyl ester standard to calibrate the column. Name % Rt(example) Rt observed 14:0ME 1 4.9 min 16:0ME 4 7.5 min 18:3ME 5 10.2 min 18:2ME 12 10.2
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