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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
, except in the presence of NBT, which gave a purple precipitate. PLAP substrates X-Phos in the absence of NBT gives a bright blue precipitate which develops slowly over time. As a result, there may be some diffusion away from the site of enzyme activity. Iron
浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
, B.E., et al., A bivalent chromatin structure marks key developmental genes in embryonic stem cells. Cell, 2006. 125(2): p. 315-26. [19] Topp, C.N., C.X. Zhong, and R.K. Dawe, Centromere-encoded RNAs are integral components of the maize kinetochore
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
, and appropriate concentrations of formamide and SSC as described in section III D.- Hypochlorite solution: 1N NaOH, 1:10 dilution of commercial bleach. - PBT: 1 x PBS, 0.1% BSA, 0.1% Triton X-100- PTw: 1 x PBS, 0.1% Tween 20- Staining solution: 100 mM
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