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- 2025年07月07日
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文献和实验Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
Experimental protocol 1. Run a blank with 1 µl of hexane. 2. Run 1 µl of fatty acid methyl ester standard to calibrate the column. Name % Rt(example) Rt observed 14:0ME 1 4.9 min 16:0ME
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
1)Plates: Long plates for sequencing and oligo preparations, medium for S1s and strand separations, short for gel shifts and restriction digests. Clean plates w/ soap & H2O, rinse w/ deion H2O, then EtOH, wipe dry. Siliconize notched
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
of fatty acid methyl ester standard to calibrate the column. Name % Rt(example) Rt observed 14:0ME 1 4.9 min 16:0ME 4 7.5 min 18:3ME 5 10.2 min 18:2ME 12 10.2
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