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- Cayman
- 27828-10 mg
- 2025年07月12日
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文献和实验Amino-allyl Reverse Transcription
with the amino-allyl dye coupling procedure all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cye dyes from coupling to free amine groups in solution. Fill one Microcon 30 concentrator with 450 ul water. Add neutralized
Revised amino-allyl labeling protocol
C indefinitely. D. Coupling Add 0.5 m L 1M sodium bicarbonate, pH 9.0 to 50 mM final. Check 1M stock solution periodically for fluctuations in pH. Monofunctional NHS-ester Cy3 (PA23001) and Cy5 dye (PA25001, Amersham) is supplied
Amino-allyl Reverse Transcription
Amino-allyl Reverse Transcription (this protocol was adapted by Joe DeRisi from one developed at Rosetta Inpharmatics) Resuspend the contents of one pack of the monofunctional NHS-ester Cy3 or Cy5 dye (mono-functional Cy3 or Cy
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