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文献和实验Replication timing by density transfer
,201-4 Sodium acetate-1,2-13 C2 99% atom purity, $242 for 1 gram Catalog # 38,937-4 D-Glucose-13 C6 99% atom purity, $193 for 1 gram Catalog # 29,928-6 Ammonium-15 N2 -sulfate 98% atom purity, $83 for 1 gram All from Sigma
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
Tris-Cl 6g Water to 2 L, pH 7.4-7.5 1xTRIzol: 200ml Final Add Guanidine thiocyanate 0.8 M FW 118.2 18.912 g Ammonium thiocyanate 0.4 M FW 76.12 6.09 g Sodium Acetate 0.1 M, pH 5 Stock 1M 20 ml Glycerol 5% 10ml Add water to 100ml Mix
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RNA
concentration of phenol is 38%. Add water to 200 ml total volume. pH ~ 4.5. Wrap with aluminum foil. 1.2xTRIzol: 200ml Final Add Guanidine thiocyanate 0.8 M x 1.2 = 0.96 M 22.694 g Ammonium thiocyanate 0.4 M x 1.2 = 0.48 M 7.308 g Sodium Acetate 0.1 M
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