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- Cayman
- 25174-500 µl
- 2025年07月16日
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文献和实验at 4°C. Transfer supernatant (nuclear fraction) to a clean microcentrifuge tube. 11. Aliquot and store at –80°C. The nuclear extracts are ready for assay. 12. Quantitate protein concentration using the Quant-iT™ Protein Quantitation Kit (Cat. no. Q
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
一 sequence beyond GenBank Gene GenBank Number(mRNA) Size(kb) GeneRacer™ Sequence Beyond GenBank Heterogeneous nuclear ribonuclear protein complex K
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