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文献和实验Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
Mandell, J.G., Falick, A.M., and Komives, E.A. 1998b. Measurement of amide hydrogen exchange by MALDI‐TOF mass spectrometry. Anal. Chem. 70:3987‐3995. Rosa, J
Co-Expression of human DNA primase p49-p58 subunits
= 7.5 Buffer B: •50mM Tris •3mM BME •1M NaCl •pH = 7.5 1.To lower the salt conc.of pooled fractions from Ni-NTA purification,dialyze O/N against Buffer A,4ºC. 2.Take dialysate and spin down to remove any particulate matter.Take supernatant and load
Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
., Morse, B., Whitman, M.M., Ivanshchenko, Y., Jaye, M., and Felder, S. 1991. Cloning of the human serotonin 5‐HT2 and 5‐HT1C receptor subtypes. Biochem. Biophys. Res. Commun. . 181:1469‐1478.
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