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文献和实验You will need: Freshly made 2M NaOH 3M sodium acetate, pH 4.5 Sterile, distilled water Absolute ethanol 70% ethanol 7 x DNA annealing buffer (280mM Tris.Cl, pH 7.5, 100mM MgCl2, 350mM NaCl) Termination mixes (40mM Tris.Cl, pH 7.5, 50mM NaCl, 10mM MgCl2, 150mM dTTP
M Tris pH 9.6 200 µl 1M MgCl2 500 µl 5 mg/ml BCIP in dimethyl formamide (BCIP= 5-bromo-4- chloro-3-indoyl phosphate, toluidine salt), US Biochemical). Store this stock at -20° wrapped in foil; it's light sensitive. 2.5 ml 2 mg/ml NBT (p-nitro blue
Planar Lipid Bilayer Experiment平面脂双层实验
on the top and connect it to the voltmeter ( while the two electrodes are in a cell with some salt solution), check to see voltage applied = what you see on voltmeter. Change battery (2 x 9V) if it?s off.2、Don?t touch calibrated knob on power box.3、Set rise time to 10
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