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文献和实验Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes
; (2) the naturally occurring fluorescent cis -parinaric acid, which specifically measures the insertion of the FA acyl chain into the hydrophobic core of the phospholipid bilayer, and (3) a fluorescein-labeled phospholipid (N-fluorescein-5-thiocarbomoyl
Synthesis of Oligonucleotide Conjugates via Aqueous Diels‐Alder Cycloaddition
of maleimide reporter groups to 5??diene?modified oligonucleotides via aqueous Diels?Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene?amidite (5??diene modifier), for the attachment of a diene group
product. The hairpin oligonucleotides (ΔG from -1.6 to -5.8 kcal/mol) are as efficient as linear primers and provide additional specificity to the PCR by preventing primer-dimers and mispriming. Self-quenched primers could be designed manually
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