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文献和实验In 2002, mitochondrial aldehyde dehydrogenase (ALDH-2) was identified as an organic nitrate bioactivating enzyme. This so-called nitrate reductase activity denitrates nitroglycerin (glycerol trinitrate) to its 1,2-glycerol dinitrate
Production of Polyclonal Antisera
All immunochemical procedures require a suitable antiserum or monoclonal antibody raised against the antigen of interest. Polyclonal antibodies are raised by injecting an immunogen into an animal and, after an appropriate time, collecting
Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies
(1 ). The aldehyde-derived adduct formation has been examined by many researchers using chemical and immunochemical methods (1 -3 ). However, on the other hand, the lipid hydroperoxide itself might react with protein as already suggested (4 ). We hypothesize
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