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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
supernatant. 29. Remove the tubes from the magnetic stand. Place on ice. 30. Wash the beads four times with 500 µL of low-salt immune complex wash buffer containing protease inhibitor cocktail. Agitate gently to resuspend
Dissociation of Cells from Primary Tissue
实验概要 Obtain single cell suspensions from primary tissue. 主要 试剂 0.25% trypsin soybean trypsin inhibitor 主要设备 stainless steel mesh [Details：100
. NOTE: EDTA is a potent inhibitor of the sequencing reaction. Template in buffer with 1 mM EDTA is acceptable. The final concentration in thesequencing reaction(20 μl) will then be 0.5 mM or less depending on the volumeof template added. A final EDTA
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