Mitoxantrone (hydrochloride)

DNA拓扑异构酶IIα和HIV-1整合酶的抑制剂（IC50s=分别为5.3和3.8μM）；在2μM浓度下在无细胞测定中插入DNA；抑制DNA合成并在体外诱导DNA-蛋白质交联；抑制K562和K562衍生的依托泊苷耐药K/VP.5细胞的生长（IC50s=分别为0.42和1.68μM）；抑制HIV-1整合酶（IC50=3.8μM）；抑制表达ACE2或细胞外结构域SARS-CoV-2刺突糖蛋白的混合HEK293T细胞群的膜融合；在0.2μM浓度下抑制SARS-CoV-2进入VeroE6细胞。An inhibitor of DNA topoisomerase IIα and HIV-1 integrase (IC50s = 5.3 and 3.8 μM, respectively); intercalates DNA in a cell-free assay at 2 µM; inhibits DNA synthesis and induces DNA-protein crosslinks in vitro; inhibits the growth of K562 and K562-derived etoposide-resistant K/VP.5 cells (IC50s = 0.42 and 1.68 µM, respectively); inhibits HIV-1 integrase (IC50 = 3.8 μM); inhibits the membrane fusion of a mixed population of HEK293T cells expressing either ACE2 or the extracellular domain SARS-CoV-2 spike glycoprotein; inhibits the entry of SARS-CoV-2 into Vero E6 cells at 0.2 µM.