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文献和实验Measuring DNA Demethylase Activity In Vitro
II or Hha I recognize subsets of CpG sequences only when the C is not methylated; thus, cleavage by this enzymes indicates demethylation of their recognition sequences (2 ) This assay is, however, obviously indirect and can only measure the state
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of ice-cold PBS containing protease inhibitor cocktail. 5. Prepare 0.2X trypsin diluted in PBS, and add 5 mL of it to the cells. Incubate in 5% CO2 for 5 min at 37°C. 6. Neutralize the trypsin with 10 mL of 10% fetal bovine
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
Geraldine Seydoux and Andrew Fire Adapted from Seydoux, G. and Fire, A. (1995). Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. In C. elegans: Modern Biological Analysis of an Organism. Methods
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