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文献和实验Phosphoamino acid analysis:Mark Kamps's method
.The blotters should be quite damp,but not soaking wet. 10.Rotate 90° counter clockwise.Electrophorese at pH 3.5,1.6 KV,13 min in the second dimension. The above electrophoresis times are appropriate when you are using a Salk/TVL/MBVL type electrophoresis rig
Phosphoamino acid analysis:Mark Kamps method
Phosphoamino acid analysis:Mark Kamps's method 1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P .
in Picro-orange 2 mins 10. Wash in water until only red blood cells are yellow 11. Stain in acid fuchsin 5 mins 12. Wash in water 13. Stain in equal parts Picro-orange and 80% alcohol 14. Differentiate in phosphomolybdic acid for 8 mins
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