CFSE Cell Division Assay Kit

Cayman公司的CFSE细胞分裂分析试剂盒提供了一种易于使用的格式，用于标记和追踪细胞在连续的细胞分裂过程中的变化。羧基荧光素二乙酸酯，琥珀酰亚胺酯（CFDA-SE）能够扩散进入细胞，在细胞内乙酸基团被切割，生成高度荧光的衍生物（CFSE），该衍生物保留在细胞中，并可通过流式细胞术检测。细胞分裂会导致荧光逐步减半，最多可以监测到八次分裂，直到荧光降低到未染色细胞的背景荧光。由于使用方便且没有细胞毒性，可以在体外或体内监测细胞分裂的过程，最长可达几周。本试剂盒包含足够的试剂，用于通过流式细胞术标记和分析最多7.5x10^8个细胞。CFSE标记的细胞还可以与任何与荧光素兼容的荧光染料进一步染色，以便用于流式细胞术。Cayman's CFSE Cell Division Assay Kit provides an easy-to-use format for labeling and tracing cells through successive cell divisions. Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) diffuses into cells, where the acetate groups are cleaved to yield a highly fluorescent derivative (CFSE) that is retained in the cell and can be detected by flow cytometry. Cell division results in sequential halving of fluorescence, and up to eight divisions can be monitored before the fluorescence is decreased to the background fluorescence of unstained cells. The ease of use and lack of cytotoxicity allows monitoring of cellular division over weeks either in vitro or in vivo. The kit contains sufficient reagents for labeling and analyzing up to 7.5x108 cells by flow cytometry. CFSE-labeled cells can be further stained with any fluorochrome compatible with fluorescein for use in flow cytometry.