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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
58 to 0.1%.Store dilution buffer at -70 degrees.Proteins are diluted in dilution buffer and quick frozen on dry ice.Thaw proteins on ice.Proteins are typically stable to multiple repeated freeze thaw.5x binding buffer 1 ml20% glycerol 400 microliters
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
CCMB buffer to make chemical competent cell
buffer 5 Measurement of competence 6 5x Ligation Adjustment Buffer 7 References 1.Preparing glassware and media Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free
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