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文献和实验PMSF (1 mM) Protease Inhibitor Cocktail, Sigma (Cat. no. P-2714) Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) Or Cell Extraction Buffer Formulation 100 mM Tris, pH 7.4 2 mM Na3 VO4 100 mM NaCl
实验试剂 1. Sterile deionized water (or TE buffer) 2. Absolute (or 95%) ethanol 实验设备 1. Protective eye-ware 实验步骤 1. MicroElute™ Gel Extraction Spin Protocol
Extraction of Nuclear Proteins
of approx 20 �m. The nuclear proteins were prepared from the purified nuclei using lysis buffer (3 ) or SDS sample buffer (4 ). The purity of the isolated nuclear fraction was evaluated by Western blot analysis using antihistone H1 antibody, a specific
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