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文献和实验Combined Biophysical and Cell-Based Approaches for the Assessment of Ligand Binding to PPAR
and quantification of ligand binding to PPARγ. This method is specific for PPARγ while ANS displays negligible interaction with other nuclear receptors such as PPARα and retinoid X receptor α (RXRα). The ANS competition assay
of a key amino acid for ligand binding and to determine the relationship between protein structure and ligand interaction. Here, we describe an application of this technique to evaluate different point mutations on the transcriptional activity of peroxisome
Ligand-Binding Assays for Collagen
Collagen is one of the most physiologically important agonists for platelet function. Two collagen-specific receptors, integrin α2 β1 and glycoprotein (GP) VI, have been identified on the platelet surface from studying patient’s platelets
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