C12 NBD Ceramide (d18:1/12:0)

C12NBD鞘氨醇（d18:1/12:0）是一种长链荧光鞘氨醇类似物，可用于测量碱性和中性鞘氨醇酶活性。它可被铜绿假单胞菌的碱性鞘氨醇酶和来自小鼠肝脏的中性鞘氨醇酶有效水解。此外，来自多种哺乳动物细胞系的碱性和中性鞘氨醇酶也能水解C12NBD鞘氨醇（d18:1/12:0）。酸性鞘氨醇酶似乎不使用C12NBD鞘氨醇（d18:1/12:0）作为底物。C12NBD鞘氨醇（d18:1/12:0）也可能被应用于鞘氨醇激酶和/或鞘磷脂合酶活性的测量。C12 NBD ceramide (d18:1/12:0) is a long-chain fluorescent ceramide analog that can be used for the measurement of alkaline and neutral ceramidase activity. It is hydrolyzed efficiently by alkaline ceramidase from P. aeruginosa and by neutral ceramidase from mouse liver. In addition, alkaline and neutral ceramidases from a variety of mammalian cell lines also hydrolyze C12 NBD ceramide (d18:1/12:0). Acid ceramidase does not appear to utilize C12 NBD ceramide (d18:1/12:0) as a substrate. The possibility exists forC12 NBD ceramide (d18:1/12:0) to also be applied to the measurement of ceramide kinase and/or sphingomyelin synthase activity.