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文献和实验Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases
of phospholipids, or by hydrolysis of diacylglycerol through the sequential action of diacylglycerol lipase and monoacylglycerol lipase (3 –6 ). These activities can be easily measured using sensitive radiometric assays as described herein.
Purification, Biochemical and Kinetic Properties of Recombinant Staphylococcus aureus Lipase
We have compared the purification procedures as well as the biochemical and kinetic properties of wild type (wt-SAL3), untagged recombinant (rec(−His)SAL3), and tagged recombinant (rec(+His)SAL3) purified forms of Staphylococcus aureus lipase
Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase
in human macrophages is more questionable, since attempts to demonstrate HSL in these cells have so far been unsuccessful (9 ). It is speculated that HSL in macrophages functions as a cholesteryl ester lipase in hydrolyzing stored cholesteryl esters
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