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- Cayman
- 10007528-100 µg
- 2025年07月16日
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
min.Warm up the rotor afterwards. 13.Collect supernatant and measure its volume.Bring GTP to 1 mM and DMSO to 10%. 14.Incubate at 37℃ for 10 min. 15.Centrifuge in a SS34 rotor at 18,000 rpm,37℃ for 20 min.Chill down the rotor afterwards. 16.Rinse
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
,collect 10 drop fractions. 13.Collect fluorescent fractions in the void volume,measure volume in a volumetric conical tube. 14.Polymerize actin by adding KCl to 100 mM and MgCl2 to 2 mM.Incubate for 30-60 min at room temperature. 15.Centrifuge in a 50Ti
Technovit® 9100 Methyl Methacrylate
as above is sufficient to destabilizes 3 - 4 liters of basis solution. Store the destabilized basis solution in portions in stoppered brown glass bottles and store either at 4°C for shorter periods or at-15°C to -20°C for longer periods of time. Preparation
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