- ￥960 - 1600
- Solarbio
- 北京
- K108039P
- 2025年07月12日
- WB IHC
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
IgG
- 亚型：
Affinity purification
- 保存条件：
Store at -20°C. Avoid freeze / thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human
- 目录编号：
3126
- 库存：
999
- 供应商：
北京索莱宝科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB IHC
- 靶点：
HLA-DRB4
- 抗体英文名：
Anti-HLA-DRB4 Polyclonal Antibody
- 抗体名：
HLA-DRB4
- 规格：
50ml/100ml
| 规格： | 50ml | 产品价格： | ￥960.0 |
|---|---|---|---|
| 规格： | 100ml | 产品价格： | ￥1600.0 |
| 英文名称 | Anti-HLA-DRB4 Polyclonal Antibody |
| 宿主 | Rabbit |
| 别名 | DR beta 4 chain |
| 稀释比例 | WB 1:1000-3000. IHC 1:100-300. |
| 蛋白分子量 | 30kDa |
| Gene ID | 3126 |
| 保存 | Store at -20°C. Avoid freeze / thaw cycles. |
| 储存液 | Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3. |
| 纯化方法 | Affinity purification |
| 亚型 | IgG |
| 免疫原 | Recombinant protein of human HLA-DRB4 |
| 亚细胞定位 | Cytoplasm? Cell membrane |
| Swiss Prot | P13762 |
| 背景资料 | Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. |
| Public Immunogen Range | 1-266aa |
| 克隆类型 | Polyclonal Antibody |
| 交叉反应 | Human |
| 应用 | WB IHC |
| 规格 | 50ul 100ul |
备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
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文献和实验Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇：make an Antibody against a Peptides 下一篇：Immunization Protocol for Monoclonal Antibody Production
Polyclonal Antibody Production
Polyclonal Antibody Production Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. Rabbit Immunization Initial Preparation Your antigen
a simple ELISA method to detect human anti-antibody response.
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